UniQ PINP for the USA
The UniQ Bone & Tissue Markers are unique serum tests for measuring collagen metabolism in bone and soft tissues.
The quantitative UniQ PINP RIA measures intact aminoterminal
propeptide of type I procollagen liberated to blood stream during bone
formation.
Generally
PINP - aminoterminal propeptide of type I procollagen - is a specific type I collagen metabolite which is liberated into the bloodstream during formation of new bone. Its concentration is directly related to the rate of bone turnover. In metabolic bone diseases such as osteoporosis, PINP is a sensitive indicator for assessing increased bone turnover in postmenopausal women. PINP is a useful parameter for monitoring the effectiveness of anti-resorptive and anabolic treatment and can be used as an aid in the management of postmenopausal osteoporosis.
UniQ PINP test provides you with
A specific and sensitive means of assessing the metabolism of bone.
PINP
- Monitoring of the effect of osteoporosis treatment
- Selecting the most effective treatment
- Monitoring patient compliance
- Changes in bone turnover seen in a couple of months.
Test results should never be used alone, without a complete clinical evaluation. Read more: UniQ PINP for the USA > Risks and limitations of the procedure
Technical data
| Products available |
|
| Use | For in vitro diagnostic use |
| Method | Radioimmunoassay |
| Sample type | Serum |
| Time to result | Appr. 4 h |
| Reading of the result | Gamma counter |
| Sensitivity | Appr. 2 µg/l |
| Specificity | 2.7% cross-reactivity with small monomeric degradation products (col1), no cross-reactivity with PIIINP. |
| Storage | 2 - 8 °C (36 - 46 °F) |
| Shel life | 6 weeks |
| Transportation | 2 - 8 °C (36 - 46 °F) |
| Size and weight | 154 (W) x 154 (L) x 105 (H), 391 g |
| Additionally needed | Gamma counter |
| Country of origin | Finland |
| Registered trademark | UniQ is a registered trademark of Aidian Oy |
About the tests
Collagen is the most common protein in the human body providing structural support to bone, skin, muscle and tendons. An indicator of type I collagen turnover, such as PINP, is useful for investigating skeletal remodelling under normal and abnormal conditions.
PINP measurements provide a sensitive tool for assessing increased bone turnover in postmenopausal women and may be particularly useful for monitoring the effect of antiresorptive and anabolic therapy on bone metabolism.
More information
UniQ PINP kit is intended to be used as an aid in the management of postmenopausal osteoporosis. Short-term changes in PINP are associated with longer-term changes in bone mineral density in postmenopausal women. Following PINP concentrations during osteoporosis treatment provides a rapid assessment of a patient’s response to osteoporosis therapy.
Documents and materials
Instructions for use
(For informative use only. Kindly always refer to the latest package insert in the kit.)
Safety Data Sheet
If you wish to receive a Safety data sheet, please contact: product.support@aidian.eu
Risks and limitations of the procedure
Results of this assay should be used in conjunction with other pertinent clinical information for making diagnostic and therapeutic decisions. Therefore, the UniQ PINP assay is not recommended for use as a screening procedure to detect the presence of osteoporosis in the general population.
When evaluating subsequent samples, collect the samples at the same time of the day, as there is a diurnal variation of PINP with the values being higher at night.
Type I collagen is also synthesised by fibroblasts and therefore can be found in loose connective tissues together with other collagen types. However, since bone is the major collagenous organ and also metabolically active throughout life, the majority of the circulating PINP is of osteoblast origin.1
PINP is metabolised in the liver.2 Severe liver disease may affect clearance from the circulation and give rise to elevated serum PINP levels.3
As with most assays utilising secondary antibodies, samples containing antibodies (such as heterophilic antibodies) that react with rabbit/goat IgG may cause erroneous results.
- Risteli J and Risteli L. Products of bone collagen metabolism. In: Seibel MJ, Robins SP, Bilezikian JP(eds). Dynamics of bone and cartilage metabolism. Academic Press, San Diego, 1999. p 275-287.
- Melkko J et al. Clearance of the aminoterminal propeptides of types I and III procollagen is a physiologicalfunction of the scavenger receptor in liver endothelial cells. J Exp Med 1994;79:405-412.
- Naylor KE and Eastell R. Measurement of biochemical markers of bone formation. In: Seibel MJ, RobinsSP, Bilezikian JP (eds). Dynamics of bone and cartilage metabolism. Academic Press, San Diego, 1999. p401-410.
Frequently asked questions about UniQ PINP for the USA
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Cartilage contains predominantly type II collagen, whereas our assays measure markers related to type I collagen metabolism. Therefore, these assays are not suitable for assessing changes in cartilage metabolism.
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The only substances in blood that could be expected to react on the PINP assay are other peptide fragments. The cross-reactivities with the UniQ PINP assay have been tested and found to be non-significant. The PINP assay only detects the intact form of the propeptide, and does not cross-react with smaller degradation fragments such as Col-1.
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We recommend using polystyrene test tubes.
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PINP is cleared from the circulation through a specialised part of the reticulo-endothelial system, the endothelial cells of the liver. These cells bind and internalise the propeptide by receptor-mediated endocytosis using so called scavenger receptors. Severe liver disease can affect the clearance of PINP. As renal insufficiency does not affect the concentration of the propeptide, it is very useful for monitoring changes in collagen metabolism in patients with renal failure.
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The molecular weight of the PINP antigen is approximately 35,000 Dalton.
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PINP is very stable in serum samples. Samples can be kept at room temperature for some days without significantly affecting the concentration of the markers and may be stored for up to 5 days at 2 - 8 °C. Storage for longer periods should be at temperatures of at least -20 °C. Repeated freezing and thawing should be avoided.
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In cell cultures of fibroblasts, PINP is produced. As the propeptide assay does not distinguish between free propeptide and propeptide still bound to the helical procollagen structure, the assay can measure both these forms of propeptide which are commonly found in cell cultures.
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The best way to process the results is to use spline function curve fitting with linear y-axis and logarithmic x-axis.